Activation of the Sarcophaga lectin gene promoter in transgenic Drosophila

Publication Type:Journal Article
Year of Publication:Submitted
Authors:T. Tanji, Kobayashi, A., Natori, S.
Volume:50
Issue:3
Pagination:131 - 138
Keywords:*Depsipeptides, *Insect Proteins, *Lectins, C-Type, *Peptides, *Promoter Regions, Genetic, *Transcriptional Activation, Animals, Animals, Genetically Modified, Anti-Bacterial Agents/immunology, beta-Galactosidase/genetics, Cell Line, Diptera/genetics/*metabolism, Drosophila melanogaster/genetics/immunology, Escherichia coli/immunology, Genes, Reporter, Lectins/*genetics, Staphylococcus aureus/immunology, Tissue Distribution, Transgenes
Abstract:

We established transgenic Drosophila strains in which the lacZ gene was expressed under the control of the 5'-upstream regulatory region of the Sarcophaga lectin gene promoter (3.1 kbp). The reporter gene was expressed in the fat bodies of the transgenic larvae when they were immunized by body pricking or treatment with Escherichia coli, which was the same as the Sarcophaga lectin gene expression in Sarcophaga larvae. However, the same reporter gene was found to be expressed constitutively in the digestive tracts of the transgenic larvae even without immunization.

Short Title:Arch Insect Biochem Physiol
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