02082nas a2200265   4500008004600000245013400046210006900180300001400249490000700263520116100270653001201431653002401443653005801467653003801525653002001563653003401583653002101617653001601638653002401654653003801678100001801716700001501734700001701749856005001766       Submitted                   eng d     00aGene expression of heat-shock proteins (Hsp23, Hsp70 and Hsp90) during and after larval diapause in the blow fly Lucilia sericata0 aGene expression of heatshock proteins Hsp23 Hsp70 and Hsp90 duri  a641 - 6470 v513 aGenes encoding heat-shock protein 23 (Hsp23), Hsp70 and Hsp90 were cloned from Lucilia sericata to examine whether their expression is related to the regulation of its larval diapause. The level of all three Hsps mRNAs was consistently low irrespective of diapause status. These results indicate that expression of Hsp23, Hsp70 and Hsp90 is not regulated in response to diapause, in contrast to the flesh fly Sarcophaga crassipalpis that shows upregulation of Hsp23 and Hsp70 and downregulation of Hsp90 during its pupal diapause. On the other hand, Hsp90 transcripts were regulated developmentally in nondiapause larvae of L. sericata, i.e., they were at low levels after cessation of feeding but were considerably upregulated a day before pupariation, suggesting that Hsp90 is involved in a developmental process that occurs between the cessation of feeding and pupariation. When diapause larvae were transferred to all of the conditions that terminate diapause and allow postdiapause development, Hsp90 transcripts were promptly upregulated. These results indicate that Hsp90 may serve as an early marker to predict diapause termination in this species.10aAnimals10aDiptera/*metabolism10aGene Expression Regulation, Developmental/*physiology10aHeat-Shock Proteins/*biosynthesis10aHot Temperature10aInsect Proteins/*biosynthesis10aLarva/metabolism10aPhotoperiod10aTissue Distribution10aTranscription, Genetic/physiology1 aTachibana, S.1 aNumata, H.1 aGoto, S., G.  uhttps://sarcophagidae.myspecies.info/node/64802191nas a2200241   4500008004600000245013100046210006900177300001400246490000700260520134400267653001201611653004601623653004501669653002501714653001101739653002201750653001001772653001601782653006901798100001701867700001501884856005001899       Submitted                   eng d     00aPossible involvement of distinct photoreceptors in the photoperiodic induction of diapause in the flesh fly Sarcophaga similis0 aPossible involvement of distinct photoreceptors in the photoperi  a401 - 4070 v553 aPhysiological characteristics of the photoreceptors involved in the photoperiodic induction of diapause were investigated in the flesh fly Sarcophaga similis. Both the early and late phases of scotophase were sensitive to light and a light pulse during each of these phases prevented diapause. Certain physiological differences between the phases were, nevertheless, detected. Compared with early scotophase, late scotophase required a light pulse with a long period and a large number of night interruption photoperiodic cycles in order to effectively prevent diapause. The diapause-averting effects of a light pulse during early scotophase were canceled by an additional long dark period, but those during late scotophase were not. Thus, the diapause-averting effects produced during early scotophase are different to those produced during late scotophase. The early scotophase was sensitive to light at wavelengths of 470nm or shorter, but not to light of 583nm or longer. In contrast, the late scotophase was sensitive to light of a broad range of wavelengths, ranging from 395 to 660nm. Furthermore, the early scotophase was considerably more sensitive to monochromatic light with low photon flux density than the late scotophase. These results suggest that different types of photoreceptor are involved in the photoperiodic response.10aAnimals10adevelopment/physiology/*radiation effects10adevelopment/physiology/radiation effects10aDiptera/*growth &amp10aFemale10aLarva/growth &amp10aLight10aPhotoperiod10aPhotoreceptor Cells, Invertebrate/*physiology/*radiation effects1 aGoto, S., G.1 aNumata, H.  uhttps://sarcophagidae.myspecies.info/node/283