02845nas a2200289   4500008004600000245020000046210006900246300001400315490000700329520183100336653001202167653002002179653003902199653003702238653003102275653002702306653003802333653002402371653001702395653002102412653002002433100001402453700001502467700001202482700001102494856005002505       Submitted                   eng d     00aComparative venom toxicity between Pteromalus puparum and Nasonia vitripennis (Hymenoptera: Pteromalidae) toward the hemocytes of their natural hosts, non-target insects and cultured insect cells0 aComparative venom toxicity between Pteromalus puparum and Nasoni  a337 - 3490 v463 aCrude venoms from two parasitoid species, Pteromalus puparum and Nasonia vitripennis (Hymenoptera: Pteromalidae) were assayed for biological activities toward hemocytes from two species of their natural hosts and eight species of their non-natural hosts as well as two lines of cultured Lepidoptera cells, respectively. By inhibiting the spreading and viability of insect hemocytes, the venom from P. puparum displayed significantly higher activities toward plasmatocytes and granular cells from both larvae and pupae of two natural hosts, Pieris rapae and Papilio xuthus, and lower activity toward those from Spodoptera litura, Musca domestica and Sarcophaga peregrina. However, no effect was found towards any type of hemocytes from other five insects tested, namely, Ectropis oblique, Galleria mellonella, Sesamia inferens, Bombyx mori and Parnara guttata. In contrast, the venom from N. vitripennis showed a narrower range of targeted insects. It appeared to have highly adverse effects on the spreading and viability of plasmatocytes and granular cells only from the natural hosts, M. domestica and S. peregrina, little toxicity to cells from P. rapae and P. xuthus, and no effect on any of the other insects tested. Pteromalus puparum venom also apparently presented a high ability to block the spreading of Tn-5B1-4 cells derived from Trichoplusia ni, and high cytotoxicity to the cells and Ha cells derived from Helicoverpa armigera. Nasonia vitripennis venom, however, only had a marked lethal effect to Ha cells. In addition, the possibility that the host range of a defined parasitoid could be assessed using our method of treating hemocytes from candidate insects with venom in vitro, and the potential of our venoms tested in the development of bio-insecticides, insect-resistant transgenic plants, are discussed.10aAnimals10aCells, Cultured10aGranulocytes/cytology/drug effects10aHemocytes/cytology/*drug effects10aHost-Parasite Interactions10aHymenoptera/*chemistry10aLepidoptera/cytology/drug effects10aSpecies Specificity10aTime Factors10aVenoms/*toxicity10aWasps/chemistry1 aZhang, Z.1 aYe, G., Y.1 aCai, J.1 aHu, C.  uhttps://sarcophagidae.myspecies.info/node/650