02479nas a2200253   4500008004600000245019500046210006900241300001400310490000800324520153600332653002901868653002201897653002801919653001201947653003501959653004101994653002302035653002502058653003302083653001702116100002002133700002202153856005002175       Submitted                   eng d     00aShifts in the carbohydrate, polyol, and amino acid pools during rapid cold-hardening and diapause-associated cold-hardening in flesh flies (Sarcophaga crassipalpis): a metabolomic comparison0 aShifts in the carbohydrate polyol and amino acid pools during ra  a753 - 7630 v1773 aFlesh flies can enhance their cold hardiness by entering a photoperiod-induced pupal diapause or by a temperature-induced rapid cold-hardening process. To determine whether the same or different metabolites are involved in these two responses, derivatized polar extracts from flesh flies subjected to these treatments were examined using gas chromatography-mass spectrophotometry (GC-MS). This metabolomic approach demonstrated that levels of metabolites involved in glycolysis (glycerol, glucose, alanine, pyruvate) were elevated by both treatments. Metabolites elevated uniquely in response to rapid cold-hardening include glutamine, cystathionine, sorbitol, and urea while levels of beta-alanine, ornithine, trehalose, and mannose levels were reduced. Rapid cold-hardening also uniquely perturbed the urea cycle. In addition to the elevated metabolites shared with rapid cold-hardening, leucine concentrations were uniquely elevated during diapause while levels of a number of other amino acids were reduced. Pools of two aerobic metabolic intermediates, fumarate and citrate, were reduced during diapause, indicating a reduction of Krebs cycle activity. Principal component analysis demonstrated that rapid cold-hardening and diapause are metabolically distinct from their untreated, non-diapausing counterparts. We discuss the possible contribution of each altered metabolite in enhancing the overall cold hardiness of the organism, as well as the efficacy of GC-MS metabolomics for investigating insect physiological systems.10a*Carbohydrate Metabolism10a*Cold Temperature10aAmino Acids/*metabolism10aAnimals10aDiptera/metabolism/*physiology10aGas Chromatography-Mass Spectrometry10aModels, Biological10aPolymers/*metabolism10aPrincipal Component Analysis10aTime Factors1 aMichaud, M., R.1 aDenlinger, D., L.  uhttps://sarcophagidae.myspecies.info/node/467