00757nas a2200193   4500008004600000245023800046210006900284300001200353490000700365100002000372700002100392700001800413700001800431700001300449700001800462700001500480700001800495856005000513       Submitted                   eng d     00aMorphological and mitochondrial DNA characters for identification and phylogenetic analysis of the myiasis-causing flesh fly Wohlfahrtia magnifica and its relatives, with a description of Wohlfahrtia monegrosensis sp. n. Wyatt & Hall0 aMorphological and mitochondrial DNA characters for identificatio  a59 - 710 v231 aHall, M., J. R.1 aAdams, Z., J. O.1 aWyatt, N., P.1 aTesta, J., M.1 aEdge, W.1 aNikolausz, M.1 aFarkas, R.1 aReady, P., D.  uhttps://sarcophagidae.myspecies.info/node/29800706nas a2200205   4500008004600000245015500046210006900201300001200270490000700282100002000289700001800309700001400327700002100341700001900362700001700381700001900398700001500417700001800432856005000450       Submitted                   eng d     00aMolecular genetic analysis of populations of Wohlfahrt ’ s wound myiasis fly, Wohlfahrtia magnifica, in outbreak populations from Greece and Morocco0 aMolecular genetic analysis of populations of Wohlfahrt s wound m  a72 - 790 v231 aHall, M., J. R.1 aTesta, J., M.1 aSmith, L.1 aAdams, Z., J. O.1 aKhallaayou, K.1 aSotiraki, S.1 aStefanakis, A.1 aFarkas, R.1 aReady, P., D.  uhttps://sarcophagidae.myspecies.info/node/25602980nas a2200241   4500008004600000020001600046245015100062210006900213260007100282300001400353490000800367520215000375100002002525700001802545700001402563700002102577700002102598700001702619700001902636700001502655700001802670856005002688       Submitted                   eng d       a{0269-283X}00aMolecular genetic analysis of populations of Wohlfahrt's wound myiasis fly, Wohlfahrtia magnifica, in outbreak populations from Greece and Morocco0 aMolecular genetic analysis of populations of Wohlfahrts wound my  b{Food Agricult Org, Unit Nat (FAO); Int Atom Energy Agency (IAEA)}  a{72 - 79}0 v{233 a{Wohlfahrt's wound myiasis fly, Wohlfahrtia magnifica (Schiner) (Diptera: Sarcophagidae), is the most important cause of traumatic myiasis in the southern Palaearctic region. Larval stages are obligate parasites and the wounds caused by infestations are very similar to those caused by Old and New World screwworm flies. During the last decade, W. magnifica appears to have expanded its range to parts of northern and central Morocco, and to Crete, Greece. Specimens of W magnifica were collected in Morocco and Crete either as larvae (preserved in 80\% ethanol) or as adults (dry-pinned). Comparison specimens were collected in Spain, Hungary and mainland Greece. A DNA fragment containing the 3'715 base pairs of the mitochondrial cytochrome b gene was amplified by polymerase chain reaction from each of 132 larvae or adults of W magnifica and the amplicons were directly sequenced and analysed phylogeographically. Twelve cytochrome b haplotypes were detected. All haplotypes from Morocco belonged to a lineage that included specimens from the Iberian peninsula, and restricted mixing of central and northern populations in Morocco was demonstrated. Cytochrome b haplotyping combined with an analysis of larval size provided clear evidence of multiple infestations of hosts in all geographical areas, with one quarter of wounds containing larvae from two to at least four females. More than 80\% of specimens from Crete contained a haplotype predominating in mainland Greece and Hungary. Our survey indicated that wohlfahrtiosis was more widespread in northern and central Morocco than previously recorded by government veterinarians. However, the prevalence of wohlfahrtiosis was low (<1 \%). The high genetic diversity of Moroccan populations is consistent with longterm endemicity, rather than recent introduction. Crete showed a higher prevalence of wohlfahrtiosis (<= 15\%) and less genetic diversity of W. magnifica, which is consistent with a recent introduction. The western and eastern Mediterranean lineages may have been isolated in different Pleistocene ice-age refugia, from which there has been limited post-glacial dispersal.}1 aHall, M., J. R.1 aTesta, J., M.1 aSmith, L.1 aAdams, Z., J. O.1 aKhallaayoune, K.1 aSotiraki, S.1 aStefanakis, A.1 aFarkas, R.1 aReady, P., D.  uhttps://sarcophagidae.myspecies.info/node/20702235nas a2200229   4500008004600000020001600046245023800062210006900300260007100369300001400440490000800454520135200462100002001814700002101834700001801855700001801873700001301891700001801904700001501922700001801937856005001955       Submitted                   eng d       a{0269-283X}00aMorphological and mitochondrial DNA characters for identification and phylogenetic analysis of the myiasis-causing flesh fly Wohlfahrtia magnifica and its relatives, with a description of Wohlfahrtia monegrosensis sp n. Wyatt \& Hall0 aMorphological and mitochondrial DNA characters for identificatio  b{Food Agricult Org, Unit Nat (FAO); Int Atom Energy Agency (IAEA)}  a{59 - 71}0 v{233 a{Wohlfahrtia magnifica (Schiner) (Diptera: Sarcophagidae) is a major cause of traumatic myiasis in livestock in Central and Eastern Europe and in countries bordering the Mediterranean. The present Study explored the utility of external body characters, genitalia characters and mitochondrial DNA characters for identification of this and related species in the subfamily Paramacronychiinae. Sequence analyses of the 3' terminal 273 bp of the mitochondrial cytochrome b gene revealed two lineages of W. magnifica, one from Spain and France and the other from the rest of Eurasia, differing by only two base pairs. Phylogenetic analysis of c cytochrome b showed that W. magnifica and Wohlfahrtia vigil Walker were sister species; this conclusion was not contradicted by a phylogenetic analysis of the morphological characters. Based on cytochrome b, the genetic distance between specimens of W. vigil from Europe and North America was sufficiently large to justify the recognition of more than one species. A new species, Wohlfahrtia monegrosensis, from northern Spain, was described, based on morphology and cytochrome b. A unique combination of external body characters of males or females were diagnostic for W. magnifica, the W. vigil group and Wohlfahrtia bella., but only the genitalia characters were diagnostic for all nine species Studied.}1 aHall, M., J. R.1 aAdams, Z., J. O.1 aWyatt, N., P.1 aTesta, J., M.1 aEdge, W.1 aNikolausz, M.1 aFarkas, R.1 aReady, P., D.  uhttps://sarcophagidae.myspecies.info/node/208