02597nas a2200289   4500008004600000245018300046210006900229300001400298490000700312520151200319653001201831653002501843653004301868653001601911653003801927653003201965653004101997653005502038653004502093653003402138100001602172700001702188700001402205700001802219700002002237856005002257       Submitted                   eng d     00aComparison of the effects of pyrokinins and related peptides identified from arthropods on pupariation behaviour in flesh fly (Sarcophaga bullata) larvae (Diptera: Sarcophagidae)0 aComparison of the effects of pyrokinins and related peptides ide  a233 - 2390 v503 aPeptides from the pyrokinin/PBAN family and some structurally related compounds identified in various arthropods were tested for acceleration of puparial contraction in flesh fly larvae. Modifications of behavioural patterns of pupariation were further studied for the active compounds using a behavioural analysis based on the recording of changes in tension of the cuticle. Nine peptides belonging to the pyrokinin/PBAN family (Lem-PK, Pea-PK-5, Lom-PK II, Hez-PBAN, Bom-DH-I), identified in five different insect species, two pyrokinin peptides derived from the genome of Drosophila melanogaster (capa-3, and hugin), and two pyrokinins identified from the white shrimp Penaeus vannamei were very active in the pupariation assay, with threshold doses within the range of 0.1-5.0 pmol larva(-1). High activity was also detected for a related peptide ETH1 from Drosophila. All of these peptides share a C-terminal PRLamide, which is essential and sufficient for the activity. Interestingly, two other structurally related peptides from Drosophila--ETH2 and capa-1--which feature conservative changes (Ile and Val, respectively) at the C-terminal Leu position, were inactive within a physiological range of concentrations. It is clear that the receptor mediating the acceleration of puparial contraction behaviour is sensitive to the introduction of greater steric bulk at the C-terminal Leu position. The peptides that accelerated pupariation showed very similar patterns of muscular and cuticular activity.10aAnimals10aArthropods/chemistry10aBiological Factors/analysis/physiology10adevelopment10aDiptera/drug effects/*growth &amp10aInsect Hormones/*physiology10aInsect Proteins/analysis/*physiology10aMetamorphosis, Biological/drug effects/*physiology10aNeuropeptides/classification/*physiology10aPupa/drug effects/growth &amp1 aZd'arek, J.1 aVerleyen, P.1 aMares, M.1 aDoleckova, L.1 aNachman, R., J.  uhttps://sarcophagidae.myspecies.info/node/765