@article {766,
	title = {Isolation and characterization of a C-type lectin cDNA specifically expressed in the tip of mouthparts of the flesh fly Sarcophaga peregrina},
	volume = {13},
	year = {Submitted},
	note = {08-10-200906-02-2012NormalPhysiology and Genetic Regulation Department, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan. mamiy3@nias.affrc.go.jpenghttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=15056360},
	pages = {133 - 140},
	abstract = {We have isolated a novel gene, CLEM 36, of the flesh fly Sarcophaga peregrina, which shows significant homology to the C-type lectin family. CLEM 36 mRNA was transcribed excessively from the second day after eclosion only in the tip of mouthparts. Whole mount in situ hybridization showed that CLEM 36 mRNA was expressed in the C-type lectin-producing tissue (CLPT) located at the entrance of the food canal and between the labellum and haustellum. Immunoblot analysis showed that the mature form of CLEM 36 protein was synthesized in the CLPT, then secreted into saliva. Our results indicate that CLEM 36 protein may play an important role in biological defence against pathogens during the food intake of this insect.},
	keywords = {*Gene Expression Profiling, Amino Acid Sequence, Animals, Base Sequence, Diptera/*genetics/immunology, DNA, Complementary/genetics, Immunoblotting, In Situ Hybridization, Lectins, C-Type/*genetics/isolation \&amp, Molecular Sequence Data, Mouth/*metabolism, purification, Reverse Transcriptase Polymerase Chain Reaction, Saliva/chemistry, Sequence Alignment, Sequence Analysis, DNA},
	author = {Yamamoto-Kihara, M. and Kotani, E.}
}
@article {292,
	title = {Gene expression responses in larvae of the fleshfly Sarcophaga bullata after immune stimulation},
	volume = {55},
	year = {Submitted},
	note = {08-10-200906-02-2012NormalInstitute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v. v. i., Prague, Czech Republic.enghttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=19545489},
	pages = {98 - 106},
	abstract = {Insect larvae develop in decaying organic matter and their defence against various microorganisms must therefore be highly efficient. In the present study, we explored the transcriptional kinetics and induction levels of eight genes in Sarcophaga bullata larvae after infection or aseptic injury. Using real-time PCR, we studied the time-dependent immune response of larvae of the fleshfly S. bullata. We compared the mRNA levels of eight selected genes in induced and non-induced larvae. The third-instar larvae of S. bullata were induced by injecting a bacterial suspension of Escherichia coli, Staphylococcus aureus or Pseudomonas aeruginosa, or by simple aseptic injury with an entomological pin. We used intact larvae as a control for basal mRNA expression. Total RNA was isolated from the whole body, fat body and haemocytes. We determined the mRNA levels of genes encoding sapecin, transferrin, prophenoloxidase 1 and 2, storage-binding protein, cathe psin L, sarcocystatin, and 26/29 kDa protease. We found that there was massive up-regulation of genes encoding the fleshfly peptide sapecin, as well as the protein transferrin. We also detected down-regulation of, or no change in, the expression of genes that encode prophenoloxidase 1 and 2, storage-binding protein, cathepsin L, sarcocystatin, and 26/29 kDa protease.},
	keywords = {*Gene Expression Regulation, Animals, Diptera/*genetics/*immunology/microbiology, Escherichia coli/immunology, Larva/genetics/immunology/microbiology, Pseudomonas aeruginosa/immunology, Reverse Transcriptase Polymerase Chain Reaction, Staphylococcus/immunology},
	author = {Masova, A. and Sindelka, R. and Kubista, M. and Kindl, J. and Jiracek, J.}
}
