@article {469,
	title = {High temperature and hexane break pupal diapause in the flesh fly, Sarcophaga crassipalpis, by activating ERK/MAPK},
	volume = {53},
	year = {Submitted},
	note = {08-10-200906-02-2012NormalDepartment of Entomology, Ohio State University, 400 Aronoff Laboratory, 318 West 12th Avenue, Columbus, OH 43210, USA. fujiwara.6@osu.eduenghttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=17681525},
	pages = {1276 - 1282},
	abstract = {Pupal diapause in the flesh fly, Sarcophaga crassipalpis, can be terminated by exposure to high temperatures or, artificially, with a topical application of organic solvents. To analyze the molecular mechanisms involved in diapause termination we explored the possibility that the mitogen-activated protein kinases (MAPK) are involved in this response. Levels of phospho-ERK increased within 10 min after hexane application. Extracellular signal-regulated kinase (ERK) was also activated when pupae were transferred from 20 to 25 degrees C, thus suggesting that ERK activation is a likely component of the signal transduction pathway used to initiate development in response to diapause-terminating signals. 20-Hydroxyecdysone and cyclic GMP terminate diapause in this fly, and the juvenile hormone analog methoprene shortens the diapause, but none of these agents activated ERK. ERK was readily activated in isolated abdomens treated with hexane, thus we conclude that ERK is directly activated by the hexane treatment. ERK activation was evident in the brain, epidermis, midgut and fat body, but not in the ventral nerve mass or ring gland, thus suggesting that ERK does not act directly on the ring gland to promote ecdysteroid synthesis but exerts its effect through stimulation of the brain.},
	keywords = {*Hot Temperature, Animals, Cloning, Molecular, Diptera/drug effects/*physiology, DNA, Complementary/genetics, Dose-Response Relationship, Drug, Ecdysterone/pharmacology, Enzyme Activation/drug effects, Extracellular Signal-Regulated MAP Kinases/genetics/*metabolism, Gene Expression Regulation, Hexanes/*pharmacology, Methoprene/pharmacology, Nucleotides, Cyclic/pharmacology, Pupa/drug effects/physiology, Signal Transduction/physiology},
	author = {Fujiwara, Y. and Denlinger, D. L.}
}
