@article {766,
	title = {Isolation and characterization of a C-type lectin cDNA specifically expressed in the tip of mouthparts of the flesh fly Sarcophaga peregrina},
	volume = {13},
	year = {Submitted},
	note = {08-10-200906-02-2012NormalPhysiology and Genetic Regulation Department, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan. mamiy3@nias.affrc.go.jpenghttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=15056360},
	pages = {133 - 140},
	abstract = {We have isolated a novel gene, CLEM 36, of the flesh fly Sarcophaga peregrina, which shows significant homology to the C-type lectin family. CLEM 36 mRNA was transcribed excessively from the second day after eclosion only in the tip of mouthparts. Whole mount in situ hybridization showed that CLEM 36 mRNA was expressed in the C-type lectin-producing tissue (CLPT) located at the entrance of the food canal and between the labellum and haustellum. Immunoblot analysis showed that the mature form of CLEM 36 protein was synthesized in the CLPT, then secreted into saliva. Our results indicate that CLEM 36 protein may play an important role in biological defence against pathogens during the food intake of this insect.},
	keywords = {*Gene Expression Profiling, Amino Acid Sequence, Animals, Base Sequence, Diptera/*genetics/immunology, DNA, Complementary/genetics, Immunoblotting, In Situ Hybridization, Lectins, C-Type/*genetics/isolation \&amp, Molecular Sequence Data, Mouth/*metabolism, purification, Reverse Transcriptase Polymerase Chain Reaction, Saliva/chemistry, Sequence Alignment, Sequence Analysis, DNA},
	author = {Yamamoto-Kihara, M. and Kotani, E.}
}
@article {44,
	title = {Utility of multi-gene loci for forensic species diagnosis of blowflies},
	volume = {11},
	year = {Submitted},
	note = {28-12-201106-02-2012Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China. zaidi_farrah@yahoo.comenghttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=21864153},
	pages = {59},
	abstract = {Contemporary studies in forensic entomology exhaustively evaluate gene sequences because these constitute the fastest and most accurate method of species identification. For this purpose single gene segments, cytochrome oxidase subunit I (COI) in particular, are commonly used. However, the limitation of such sequences in identification, especially of closely related species and populations, demand a multi-gene approach. But this raises the question of which group of genes can best fulfill the identification task? In this context the utility of five gene segments was explored among blowfly species from two distinct geographic regions, China and Pakistan. COI, cytochrome b (CYTB), NADH dehydrogenase 5 (ND5), nuclear internal transcribed spacers (ITS1 and ITS2), were sequenced for eight blowfly species including Chrysomya megacephala F. (Diptera: Calliphoidae), Ch. pinguis Walker, Lucilia sericata Meigen L. porphyrina Walker, L. illustris Meigen Hemipyrellia ligurriens Wiedemann, Aldrichina grahami Aldrich, and the housefly, Musca domestica L. (Muscidae), from Hangzhou, China; while COI, CYTB, and ITS2 were sequenced for four species, i.e. Ch. megacephala, Ch. rufifacies, L. cuprina, and the flesh fly, Sarcophaga albiceps Meigen (Sarcophagidae), from Dera Ismail Khan Pakistan. The results demonstrate a universal utility of these gene segments in the molecular identification of flies of forensic importance.},
	keywords = {Animals, Cytochromes b/genetics, Diptera/chemistry/*classification/genetics, DNA, Intergenic/chemistry, DNA, Mitochondrial/*chemistry, Electron Transport Complex IV/genetics, Forensic Sciences/*methods, Genes, Insect, Genetic Variation, NADH Dehydrogenase/genetics, Sequence Analysis, DNA, Species Specificity},
	author = {Zaidi, F. and Wei, S. J. and Shi, M. and Chen, X. X.}
}
