@article {401,
	title = {The hsp27 gene of the Mediterranean fruit fly, Ceratitis capitata: structural characterization, regulation and developmental expression},
	volume = {17},
	year = {Submitted},
	note = {In the present study, a genomic DNA clone encoding the medfly homolog of Drosophila melanogaster hsp27 gene, named Cchsp27, was isolated. We sequenced a part of the clone containing the coding region, the 5{\textquoteright} untranslated region and approximately 2.8 Kb of the 5{\textquoteright} flanking region of the gene. Phylogenetic analysis of several insect small heat shock proteins, suggested that CcHsp27 is orthologous to Drosophila Hsp27 and Sarcophaga crassipalpis Hsp25. The Cchsp27 gene was mapped at the 81A division of the sixth chromosome which coincides with one of the major heat shock puffs of medfly. Structural analysis of the 5{\textquoteright} flanking region of the Cchsp27 gene revealed the presence of five putative heat shock elements and one putative ecdysone response element. In addition to heat induction, the Cchsp27 gene was expressed at several stages of normal medfly development. In general, the developmental expression pattern of the Cchsp27 gene was similar to the respective pattern of Drosophila hsp27 gene. However, there were some important differences in certain developmental stages suggesting differential regulation of the hsp27 gene in the two dipterans species. Salivary gland culture experiments showed that the Cchsp27 gene is regulated by 20-hydroxyecdysone29-09-200906-02-2012Normal},
	pages = {699 - 710},
	author = {Kokolakis, G. and Tatari, M. and Zacharopoulou, A. and Mintzas, A. C.}
}
@article {288,
	title = {Two hsp23 genes in the Mediterranean fruit fly, Ceratitis capitata: structural characterization, heat shock regulation and developmental expression},
	volume = {18},
	year = {Submitted},
	note = {08-10-200906-02-2012NormalDepartment of Biology, University of Patras, Greece.enghttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=19320758},
	pages = {171 - 181},
	abstract = {In the present study, we characterized a 3320-bp genomic DNA fragment encoding two medfly (Ceratitis capitata) homologues of the Drosophila melanogaster heat shock protein 23 (hsp23) gene, named Cchsp23-alphaand -beta. The two medfly hsp23 genes are transcribed in opposite directions and encode two almost identical proteins. Furthermore, the two genes exhibit a very high degree of similarity in their 5{\textquoteright} untranslated and proximal promoter regions. Phylogenetic analysis indicated that the CcHsp23 proteins are orthologous to Drosophila Hsp23 and Sarcophaga crassipalpis Hsp23. Structural analysis of the 5{\textquoteright} flanking regions of the Cchsp23 genes revealed the presence of several putative heat shock elements. Both CcHsp23 genes are induced by heat in a similar manner. In addition to heat-induction, the Cchsp23 genes are expressed at several stages of normal development as well as in ovaries and testes. In general, the developmental expression patterns of the medfly genes are similar, suggesting that they are under similar regulatory mechanisms. However, the expression of the Cchsp23 genes differs significantly from the expression of the Drosophila hsp23 gene in certain embryonic and larval stages, suggesting differential regulation of the hsp23 genes in the two dipteran species. The expression of both Cchsp23 genes in adult flies is increased with age, especially in males.},
	keywords = {*Gene Expression Regulation, Developmental, *Genes, Insect, Amino Acid Sequence, Animals, Base Sequence, Binding Sites, Ceratitis capitata/*genetics, Drosophila, Heat-Shock Proteins/chemistry/*genetics/metabolism, Heat-Shock Response/*genetics, Molecular Sequence Data, Phylogeny, Restriction Mapping, Sequence Alignment},
	author = {Kokolakis, G. and Kritsidima, M. and Tkachenko, T. and Mintzas, A. C.}
}
